IPC PAS Articles

Displaying 1 - 5 of 235 records

Cationic−anionic complexes of Cu(II) and Co(II) with N-scorpionate ligand – structure, spectroscopy, and catecholase activity
(Royal Society of Chemistry, 2025) Zienkiewicz-Machnik, Małgorzata; Luboradzki, Roman; Mech-Piskorz, Justyna; Angulo, Gonzalo; Nogala, Wojciech; Ratajczyk, Tomasz; Aleshkevych, Pavlo; Kubas, Adam; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland
We report structural and physicochemical characterization supported by quantum chemical studies of two novel copper(II) [CuLCl]2[CuCl4] (1) and cobalt(II) [CoLCl][CoL′Cl3] (2) cationic−anionic complexes with N-scorpionate type ligand, N,N,N-tris(3,5-dimethylpyrazol-1-ylmethyl)amine (L), where L′ is 1-methylamine-3,5-dimethylpyrazole. The obtained complexes are the first reported examples of cationic−anionic coordination compounds tested for catecholase activity. Interestingly, only copper complex (1) shows catalytic activity in the oxidation of 3,5-di-tert-butylcatechol (3,5-DTBC), which turned out to be solvent dependent. Here, experimental UV-vis spectroscopy of 1 shows that essential features of the solid-state spectrum are maintained in DMSO and MeOH solvents. In contrast, the build-up of a new feature around 465 nm for 1 in CH3CN was noted, along with negligible activity. According to quantum chemical calculations, this feature could be attributed to ligand-to-metal excitations within the [CuCl4]2− fragment disturbed by adjacent [CuLCl]+ species. The band shifts to lower energies compared to solid-state measurements as the two charged fragments get closer due to Coulomb interactions. In DMSO, the solvent molecule serves as an inert ligand in a [CuLCl]+ fragment and blocks the catalytic center, disturbing the formation of the [catalyst–substrate] complex and decreasing activity, while in MeOH, the solvent effectively stabilizes [CuCl4]2−via a H-bond network and the free coordination site is accessible, thus allowing a substrate molecule to bind. The critical advantage of the investigated complexes, in the context of their possible catalytic activity, was the fact that their usage would not introduce any unnecessary counterions.
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations
(Nature Portfolio, 2024-11-28) Hołubowicz, Rafał; Du, Samuel W.; Felgner, Jiin; Smidak, Roman; Choi, Elliot H.; Palczewska, Grazyna; Rodrigues Menezes, Carolline; Dong, Zhiqian; Gao, Fangyuan; Medani, Omar; Yan, Alexander L.; Hołubowicz, Maria W.; Chen, Paul Z.; Bassetto, Marco; Risalit,i Eleonora; Salom, David; Workman, J. Noah; Kiser, Philip D.; Foik, Andrzej T.; Lyon, David C.; Newby, Gregory A.; Liu, David R.; Felgner, Philip L.; Palczewski, Krzysztof; Cheifet Barbara; Almeida Filipe; Caprettini Valeria; Griffo Alessandra; Haskell Jennifer; Gavin Herbert Eye Institute – Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, CA, USA; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wroclaw, Poland; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA; Adeline Yen Mah Vaccine Center, Department of Physiology and Biophysics, University of California, Irvine, CA, USA; Program in Neuroscience, Amherst College, Amherst, MA, USA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA; Research Service, Tibor Rubin VA Long Beach Medical Center, Long Beach, CA, USA; Department of Genetic Medicine, Johns Hopkins University, Baltimore, MD, USA; Department of Clinical Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of California, Irvine, CA, USA; International Centre for Translational Eye Research (ICTER); Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland; Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, CA, USA; Department of Chemistry, University of California, Irvine, CA, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA
Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses encoding for Cas9 and its respective guide RNA. However, transient RNP activity does not typically lead to optimal editing outcomes. Here we show that the efficiency of delivering RNPs can be enhanced by cell-penetrating peptides (covalently fused to the protein or as excipients) and that lipid nanoparticles (LNPs) encapsulating RNPs can be optimized for enhanced RNP stability, delivery efficiency and editing potency. Specifically, after screening for suitable ionizable cationic lipids and by optimizing the concentration of the synthetic lipid DMG-PEG 2000, we show that the encapsulation, via microfluidic mixing, of adenine base editor and prime editor RNPs within LNPs using the ionizable lipid SM102 can result in in vivo editing-efficiency enhancements larger than 300-fold (with respect to the delivery of the naked RNP) without detectable off-target edits. We believe that chemically defined LNP formulations optimized for RNP-encapsulation stability and delivery efficiency will lead to safer genome editing.
Multiwavelength laser doppler holography (MLDH) in spatiotemporal optical coherence tomography (STOC-T) for human retinal blood flow visualization in vivo
(Elsevier, 2024-03-12) Borycki, Dawid; Auksorius, Egidijus; Węgrzyn, Piotr; Liżewski, Kamil; Tomczewski, Sławomir; Žičkienė, Ieva; Adomavičius, Karolis; Karnowski, Karol; Wojtkowski, Maciej; International Centre for Translational Eye Research, Warsaw, Poland; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland; Center for Physical Sciences and Technology (FTMC), Vilnius, Lithuania; Faculty of Physics, University of Warsaw, Warsaw, Poland
Spatiotemporal optical coherence tomography (STOC-T) is the new modality for high-speed, crosstalk- and aberration-free volumetric imaging of biological tissue in vivo. STOC-T extends the Fourier-Domain Full-Field Optical Coherence Tomography (FD-FF-OCT) by the spatial phase modulation that enables reduction of spatial coherence of the tunable laser, which suppresses coherent noise, and consequently, improves the imaging depth. Furthermore, the geometrical aberrations are removed computationally in postprocessing. We recently demonstrated high-speed, high-resolution STOC-T of the human eye in vivo. Here, we show that the dataset produced by STOC-T can be processed differently to reveal blood flow in the superficial and deep retina layers. To render the blood flow, we estimated the spectral broadening to intrinsic sample motion. This approach enables us to access the approximated information about the Doppler-shifted optical field backscattered from the sample. This novel approach enables us to access the approximated information about the Doppler-shifted optical field backscattered from the sample and analyze it using methods from the Laser Doppler Holography (LDH). By doing so, we can render the blood flow images from a single volume. Our method, denoted as multiwavelength laser Doppler holography (MLDH) links LDH and laser Doppler flowmetry with the multiwavelength holographic tomography to enable noninvasive visualization and quantification of the blood flow deep into the human retina at high speeds and high transverse resolution in vivo.
Chirped flicker optoretinography for in vivo characterization of human photoreceptors’ frequency response to light
(Optica Publishing Group, 2024-04-30) Tomczewski, Sławomir; Wegrzyn, Piotr; Wojtkowski, Maciej; Curatolo, Andrea; International Centre for Translational Eye Research, Warszawa, Poland; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland
Flicker electroretinography (ERG) has served as a valuable noninvasive objective tool for investigating retinal physiological function through the measurement of electrical signals originating from retinal neurons in response to temporally modulated light stimulation. Deficits in the response at certain frequencies can be used as effective biomarkers of cone-pathway dysfunction. In this Letter, we present the progress we made on its optical counterpart—photopic flicker optoretinography (f-ORG). Specifically, we focus on the measurement of the response of light-adapted retinal photoreceptors to a flicker stimulus with chirped frequency modulation. In contrast to measurements performed at discrete frequencies, this technique enables a significantly accelerated characterization of photoreceptor outer segment optical path length modulation amplitudes in the nanometer range as a function of stimulus frequency, enabling the acquisition of the characteristic frequency response in less than 2 sec.
Optical Coherence Tomography Imaging by a Fully Integrated MOEMS Endomicroscopy Probe With Mirau Microinterferometer and Two-Axis Electrothermal Microscanner Using Lissajous Trajectory Scanning
(IEEE Council, 2024-05-01) Struk, Przemysław; Bargiel, Sylwester; Józwik, Michal; Mirecki, Bartosz; Wojtkowski, Maciej; Xie, Huikai; Gorecki, Christophe; Department of Optoelectronics, Silesian University of Technology, Gliwice, Poland; FEMTO-ST Institute, Besançon cedex, France; Faculty of Mechatronics, Institute of Micromechanics and Photonics, Warsaw University of Technology, Warsaw, Poland; ICTER - International Centre for Translational Eye Research, Warsaw, Poland; Department of Electrical and Computer Engineering, University of Florida, Gainesville, USA; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland; Beijing Institute of Technology, Beijing, China
Abstract—This article presents the results of a study focused on the development of an integrated probe intended for endomicroscopic application. A new type of transverse scanning probe is described, which was built using Mirau microinterferometer fabricated in microopto- electromechanical systems (MOEMS) technology, and a two-axis electrothermal actuator fabricated in micro-electromechanical systems (MEMS) technology, connected with a gradient-index (GRIN) lens collimator and single-mode fiber. Herein, we present a numerical analysis and an optimization of endomicroscopic probe-scanning properties, based on Lissajous curves. The key part of this article is 2-D and 3-D imaging of phantom structures, based on polymer material, light-scattering material, and USAF-target pattern visualized with an endomicroscopy probe. The imaging was obtained by the swept source optical coherence tomography (SS-OCT) technique, working at a central wavelength λc = 1060 nm, a swept range 1λ = 100 nm, an A-scan rate fa = 200 kHz, and the scanning of samples with the use of Lissajous curves.

Recent Submissions