Conference papers

Displaying 1 - 5 of 574 records

Polioksometalany pirydyniowe - nowoczesne katalizatory w procesie utleniania
(Politechnika Poznańska, 2025) Wieszczycka, Karolina; Namińska, Julia; Banach, Weronika; Piotr Michałowski; Politechnika Poznańska, Wydział Technologii Chemicznej, Instytut Technologii i Inżynierii Chemiczne; Centrum Biologii Chemicznej, Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Innowacyjne nanomateriały do oksydacyjnego odsiarczania
(Wydział Technologii Chemicznej Politechniki Poznańskiej, 2025) Namińska,Julia; Collura, David; Almarmadh, Ali; Nowicki, Marek; Buchwald Zuzanna; Wieszczycka, Karolina; Politechnika Poznańska, Wydział Technologii Chemicznej, Instytut Technologii i Inżynierii Chemicznej; Politechnika Poznańska, Wydział Inżynierii Materiałowej i Fizyki Technicznej, Instytut Fizyki
EDITRANSOR - Employee-Driven Innovation evolving into Organizational Routine
(LUT Scientific and Expertise Publications, 2024-06) Padzik-Wołos, Agnieszka; Akademia Leona Koźmińskiego
The discourse on Employee-Driven Innovation (EDI) centers on the initial stages of the EDI process: idea generation, evaluation and implementation. However, a research gap exists concerning the internal structure of the EDI process - specifically, its phases and influencing factors. In this paper, the phenomenon of EDITRANSOR – transition of EDI into Organizational Routine (OR) is presented. Preliminary findings, drawn from two distinct cases, center on exploring the internal structure of EDITRANSOR and the factors that influence it. By employing a case study method, the research extends the understanding of the EDI process in three significant ways. Firstly, it highlights the EDITRANSOR phases, with the unexplored phase of EDI solidification into OR. Secondly, it demonstrates categories of factors enabling and impeding the process. Thirdly, it suggests that factors vary across EDITRANSOR phases. Research findings hold practical value, allowing organizations with ongoing or planned EDI programs to update their policies accordingly.
Evaluation of 3D-printed microneedles for potential use in the treatment of depression
(APGI, APV and SITELF (Association de Pharmacie Galénique Industrielle / International Society of Drug Delivery Sciences and Technology; Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e. V. / International Association for Pharmaceutical Technology and Industrial Pharmacy; SITELF Italian Association of Pharmaceutical Technology and Legislation), 2026-03-26) Wojtyłko, Monika; Froelich, Anna; Jadach, Barbara; Kuczko, Wiesław; Wichniarek, Radosław; Mlynarczyk, Dariusz T.; Budna-Tukan, Joanna; Białek, Antoni; Osmałek, Tomasz; APGI, APV and SITELF (Association de Pharmacie Galénique Industrielle / International Society of Drug Delivery Sciences and Technology; Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e. V. / International Association for Pharmaceutical Technology and Industrial Pharmacy; SITELF Italian Association of Pharmaceutical Technology and Legislation); 3D Printing Division, Chair and Department of Pharmaceutical Technology, University of Medical Sciences, Poland; Doctoral School, Poznan University of Medical Sciences, Poland; Division of Industrial Pharmacy, Chair and Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poland; Institute of Materials Technology, Faculty of Mechanical Engineering, Poznan University of Technology, Poland; Chair and Department of Chemical Technology of Drugs, University of Medical Sciences, Poland; Department of Immunology, Poznan University of Medical Sciences, Poland; Department of Anatomy and Histology, Collegium Medicum, University of Zielona Gora, Poland; Student’s Research Group of Pharmaceutical Technology, The Student Scientific Society of Poznan University of Medical Sciences, Poland; Chair and Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poland; School of Pharmacy, Queen’s University Belfast, UK
INTRODUCTION Agomelatine is an antidepressant used to treat major depressive episodes in adults. Currently, it is only available on the market as an oral tablet, but its bioavailability after oral administration is less than 5% [1] due to the extensive liver metabolism. Finding an alternative route of administration for this substance can result in an increased bioavailability and smaller doses of the drug necessary to achieve a therapeutic effect, which in turn translates into a reduced risk of side effects and more effective therapy. This study aimed to develop microneedles as a delivery system for transdermal administration of agomelatine. The use of microneedle systems is intended to overcome the stratum corneum barrier and facilitate the penetration of the drug into the dermis, from where it can be absorbed into the bloodstream. Microneedles were manufactured using 3D-printing, which is being intensively researched for medical applications and ensures fast and efficient production of systems, and the possibility of personalizing the devices. Two 3D-printing techniques: PolyJet and Masked Stereolithography were used. Agomelatine was introduced into the systems directly and in the form of a coating from a previously prepared gel. The microneedles were subjected to permeation test on human skin ex vivo, penetration test, and were also examined for stability and toxicity to determine the most suitable type for in vivo studies. MATERIALS AND METHODS Microneedle systems were manufactured in a 3D-printing process, using PolyJet method and Masked Stereolithography (MSLA) method, both of which involve curing photosensitive resin with UV light. The initial design of microneedle systems differed in shape, length, and the number of needles on the base. Microneedles manufacturing and coating Microneedle systems were printed using Sonic Mini 8K Phrozen (Hsinchu, Taiwan) printer for MSLA method, and Stratasys J5 (Stratasys Ltd., Eden Prairie, MN, USA) for PolyJet method. In both cases, biocompatible printing material was used. Initially, four geometries of microneedles were printed (cones, pyramids, rockets, and spearheads). Two methods of drug loading were applied. The first involved adding the drug dissolved in ethanol to the resin before printing. The second was coating with the gel containing agomelatine, either dissolved in ethanol or in the form of a suspension. Ex vivo permeation study Permeation studies were performed using full-thickness human skin (Biopredic International, France) and Franz diffusion cells (Teledyne Hanson Research, USA). Samples of the skin with microneedles were mounted on Franz diffusion cells filled with Phosphate Buffered Saline (PBS) pH=7,4 with the addition of sodium azide (0,02% w/v). The study was conducted over 7 days. Samples of acceptor fluid were taken at specified time points and replaced with a fresh medium. The samples were analyzed using High Performance Liquid Chromatography (Shimadzu, Nexera, Japan). Insertion study Insertion test was performed with the use of a Shimadzu AGS-X texture analyzer (Shimadzu, Kyoto, Japan) and TrapeziumX 1.52 software (Shimadzu, Japan). The microneedle system was mounted on the cylindrical sensor placed above 10 layers of laboratory Parafilm® (1270 µm), situated on a metal table. The sensor moved downward at 0.05 mm/s until the force of 32 N [2], and then was held in place for 30 seconds. The number of holes in each layer of Parafilm® M was calculated. Additionally, for Pyramid geometry, microneedles were inserted into a full-thickness human skin sample. Then, the sample was frozen at -80°C, cut using the cryomicrotome (Leica Microsystems, CM1850-1-1, Nussloch, Germany), and investigated under a microscope to confirm the epidermis disruption. Stability studies The stability and photostability of the prepared samples were determined using a temperature and humidity chamber with illumination, TH-ICH-800 (Jeio Tech Co., Ltd., Daejeon, Republic of Korea), according to the ICH guidelines Q1A and Q1B. Microtox acute toxicity study The toxicity of the microneedles was evaluated following the procedure of the 81.9% Screening test with slight modifications [3]. Microtox acute reagent (lyophilized Aliivibrio fischeri bacteria; ModernWater plc, London, United Kingdom) was rehydrated, diluted tenfold with Microtox Diluent, and incubated at 15°C for 15 minutes. Then the bioluminescence of the Aliivibrio fischeri bacterial suspension was measured, and the microneedle system sample was added. Changes in bioluminescence were measured after 5 and 15 minutes. RESULTS Both printing methods proved effective in obtaining good resolution of microneedles with different geometries, but only the MSLA method allowed the drug to be introduced into the matrix by adding it to the resin before printing. In contrast, both methods were successful in applying agomelatine to the microneedle systems by coating [Figure 1]. Figure 1. Microneedle coating process utilizing dedicated tooling. The permeation study showed that the drug passes into the acceptor fluid from all tested formulations, which indicates effective skin barrier penetration. In addition, microneedle systems containing agomelatine incorporated into the system matrix (“MIX” type) showed a nearly linear relationship between the amount of drug released and time. Considering the MSLA method, the rocket geometry was prone to breakage, and the Spearhead geometry required more force to penetrate than the cones, despite having the same number of needles per base. Pyramid geometry, which performed worst in the test using Parafilm®, was additionally tested on a sample of full-thickness human skin [Figure 2], and proved its efficiency. Of the two methods, PolyJet microneedles proved to be more effective at penetrating Parafilm®. Figure 2. The photo of skin cross-section with visible holes in the epidermis of the human skin sample. Stability studies indicated a decrease in the amount of agomelatine in most samples at temperature 40±2°C and 75±5% humidity over a period of up to 3 months. However, no clear influence of geometry or gel type on photostability was found. MSLA method showed lower toxicity than PolyJet in some cases, and drug loading did not cause an increase in the toxic effect in this method. CONCLUSIONS Both printing methods proved to be accurate, but only MSLA allowed the drug to be loaded directly into the system matrix. Moreover, the need for packaging that protects against external factors was demonstrated. Penetration tests allowed Pyramids and cones to be selected as the most promising geometries for further research. Also, for MSLA systems, AGM did not increase the toxicity of the samples in the Microtox® test. REFERENCES B. Green, Focus on agomelatine, Current Medical Research and Opinion 27 (2011) 745–749. J. Leanpolchareanchai, N. Nuchtavorn, Response Surface Methodology for Optimization of Hydrogel-Forming Microneedles as Rapid and Efficient Transdermal Microsampling Tools, Gels 9 (2023) 306. B. Czarczynska-Goslinska, T. Goslinski, A. Roszak, A. Froelich, P. Szyk, D.T. Mlynarczyk, L. Sobotta, I. Budnik, O. Kordyl, T. Osmałek, Microneedle System Coated with Hydrogels Containing Protoporphyrin IX for Potential Application in Pharmaceutical Technology, MPs 7 (2024) 73.
Ex vivo permeation testing of 3d-printed microneedles for depression using human skin
(Jagiellonian University Medical College, 2024-09-18) Wojtyłko, Monika; Lamprou, Dimitrios A.; Froelich, Anna; Jadach, Barbara; Kuczko, Wiesław; Wichniarek, Radosław; Szybowicz, Mirosław; Nowicka, Ariadna B.; Kordyl, Oliwia; Krysztofiak, Julia; Białek, Antoni; Osmałek, Tomasz; Chair and Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poland; Doctoral School, Poznan University of Medical Sciences, Poland; School of Pharmacy, Queen’s University Belfast, UK; Institute of Materials Technology, Faculty of Mechanical Engineering, Poznan University of Technology, Poland; Institute of Materials Research and Quantum Engineering, The Faculty of Materials Engineering and Technical Physics, Poznan University of Technology, Poland
INTRODUCTION Emerging technologies such as 3D printing are intensively investigated regarding therapy personalization. The flexibility of that technology enables the manufacturing of dosage forms and drug delivery systems in different sizes and shapes. 3D printing is one of the methods for obtaining microneedle systems. Microneedles are tiny needles, usually placed on a joint base. Thanks to the sharp tip and optimal geometry, they can pierce the skin and deliver the drug without causing discomfort. Moreover, physically crossing the stratum corneum barrier can improve drug delivery efficiency. Agomelatine is an antidepressant with very low bioavailability after oral administration due to the first-pass effect. Delivering agomelatine transdermally using a microneedle system could potentially improve its bioavailability and reduce doses during the therapy. The study aimed to compare different 3D-printed microneedle systems containing agomelatine regarding drug permeation ability through the human skin ex vivo. The systems differed in size, shape, and drug loading method, which could impact the permeation rate and profile. MATERIALS AND METHODS Microneedle systems were obtained using a 3D printing method based on curing the photosensitive resin with UV light. Two methods of drug loading were applied: either coating with a gel containing the drug or incorporation of the substance into the system at the stage of manufacturing. Two types of geometries (pyramids and cones) were used. Permeation studies Permeation studies were performed using Franz diffusion cells (Teledyne Hanson Research, USA) and full-thickness human skin. The skin was obtained from patients who had undergone surgical operations (Biopredic International, France). Samples of the skin with microneedles were placed on Franz diffusion cells. Phosphate Buffered Saline (PBS) pH=7,4 was used as the acceptor media. Due to the prolonged experiment time (7 days), sodium azide (0,02% w/v) was added to the PBS as an antimicrobial agent. During the study, samples of acceptor fluid were taken at specified time points and immediately replaced with a fresh medium. The samples were analysed using HPLC (Shimadzu, Nexera, Japan). Determining the amount of drug in tissue After the experiment, pieces of the skin were cut and placed in the homogenizing tubes. The mixture of water and ethanol was added to the tubes. Samples were homogenized and centrifuged. The supernatant was filtered and drug amount was analyzed. RESULTS AND DISCUSSION The full-thickness human skin used in the experiment mimicked the natural skin barrier. It allowed for the assessment of whether the systems were sharp enough to be inserted into the skin during application. Obtained microneedle systems were suitable for piercing the skin. Agomelatine released from the microneedles penetrated through the skin to the acceptor fluid, which can potentially simulate transdermal delivery. However, some of the drug was held in the skin tissue. All formulations were investigated for seven days to obtain a complete view of the permeation profile. Depending on the drug loading method, the release profile differed. The microneedle systems with drug incorporated at the manufacturing stage tended to show linear correlation between cumulative drug amount in the acceptor fluid and time. However, in the case of a coated system, the release showed a two-stage profile characterized by faster release at the beginning and linear correlation at the second stage. Microneedle systems obtained by the same 3D printing technique and loaded using the same method showed various release rates, probably due to the difference in microneedle geometry. CONCLUSIONS The study on Franz diffusion cells using full-thickness human skin ex vivo allowed for comparing the permeation abilities of various 3D-printed microneedle systems. Microneedles delivered the drug through the skin into the acceptor fluid. Depending on the drug loading method, the systems differed in their permeation profile. Still, in both cases, it was a slow process lasting over seven days, which indicates the potential for sustained drug delivery. The geometry of the microneedle system did not affect the permeation profile but impacted the system’s loading capacity. Funding source: The work was performed as a result of the research project no. 2021/42/E/NZ7/00125 (ID: 526262) financed by the National Science Centre (Poland).

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