Changes in the mitochondrial membrane potential in endothelial cells can be detected by Raman microscopy

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dc.contributor.authorPieczara, Anna
dc.contributor.authorMatuszyk, Ewelina
dc.contributor.authorSzcześniak, Piotr
dc.contributor.authorMłynarski, Jacek
dc.contributor.authorBarańska, Małgorzata
dc.contributor.organizationJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian Universityen
dc.contributor.organizationFaculty of Chemistry, Jagiellonian Universityen
dc.contributor.organizationInstitute of Organic Chemistry, Polish Academy of Sciencesen
dc.date.accessioned2022-10-20T11:47:14Z
dc.date.available2022-10-20T11:47:14Z
dc.date.issued2022
dc.description.abstractThe role of mitochondria goes beyond their capacity to create molecular fuel and includes e.g. the production of reactive oxygen species and the regulation of cell death. In endothelial cells, mitochondria have a significant impact on cellular function under both healthy and pathological conditions. Endothelial dysfunction contributes to the development of various lifestyle diseases and the key players in their pathogenesis are among others vascular inflammation and oxidative stress. The latter is very closely related to mitochondrial dysfunction; however, it is not straightforward. First, because mitochondria are small cellular structures, and second, it requires a sensitive method to follow the subtle biochemical changes. For this purpose, Raman microscopy (RM) was used here, which is considered a high-resolution method and can be applied in situ, usually as a non-labeled technique. In this work, we show that RM can not only locate mitochondria in the cell but also track their functional changes. Moreover, we test if labeling cells with Raman probes (Rp) can improve the specificity and sensitivity of RM (compared to conventional labeled techniques such as fluorescence, and the non-labeled Raman technique). MitoBADY Rp was used to detect changes in mitochondrial membrane potential as an indicator of mitochondrial activity, e.g. hyperpolarization or distortion of the proton gradient in the intermembrane space (depolarization). Thus, we show and compare RM, in the form of a label and non- labeled, to such a subtle cellular analysis.en
dc.description.sponsorshipNational Science Center: OPUS15 no. UMO-2018/29/B/ST4/00335
dc.identifier.citationA. Pieczara, E. Matuszyk, P. Szcześniak, J. Mlynarski, M. Baranska, Changes in the mitochondrial membrane potential in endothelial cells can be detected by Raman microscopy, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2022), doi: https://doi.org/10.1016/j.saa.2022.121978en
dc.identifier.doi10.1016/j.saa.2022.121978
dc.identifier.issn1386-1425
dc.identifier.issn1873-3557
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/21761
dc.language.isoen
dc.publisherElsevieren
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectmitochondriaen
dc.subjectcytochrome cen
dc.subjectRaman microscopyen
dc.subjectRaman probeen
dc.subjectMitoBADYen
dc.subjecthormonesen
dc.titleChanges in the mitochondrial membrane potential in endothelial cells can be detected by Raman microscopyen
dc.typearticleen
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