Physicochemical Properties Predict Retention of Antibiotics in Water-in-Oil Droplets

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dc.contributor.authorRuszczak, Artur
dc.contributor.authorJankowski, Paweł
dc.contributor.authorVasantham, Shreyas
dc.contributor.authorScheler, Ott
dc.contributor.authorGarstecki, Piotr
dc.contributor.organizationInstitute of Physical Chemistry, Polish Academy of Sciencesen
dc.contributor.organizationDepartment of Chemistry and Biotechnology, Tallinn University of Technology (TalTech), Estoniaen
dc.date.accessioned2023-01-23T18:21:26Z
dc.date.available2023-01-23T18:21:26Z
dc.date.issued2023-01-09
dc.description.abstractWater-in-oil droplet microfluidics promises capacity for high-throughput single-cell antimicrobial susceptibility assays and investigation of drug resistance mechanisms. Every droplet must serve as an isolated environment with a controlled antibiotic concentration in such assays. While technologies for generation, incubation, screening, and sorting droplets mature, predictable retention of active molecules inside droplets remains a major outstanding challenge. Here, we analyzed 36 descriptors of the antibiotic molecules against experimental results on the cross-talk of antibiotics in droplets. We show that partition coefficient and fractional polar surface area are the key physicochemical properties that predict antibiotic retention. We verified the prediction by monitoring growth inhibition by antibiotic-loaded neighboring droplets. Our experiments also demonstrate that transfer of antibiotics between droplets is concentration- and distance-dependent. Our findings immediately apply to designing droplet antibiotic assays and give deeper insight into the retention of small molecules in water-in-oil emulsions.en
dc.description.sponsorshipNational Science Centre, Poland, 2018/30/A/ST4/00036, Maestro 10. Estonian Research Council grants MOBTP109 and PRG620 and from the TTU Development Program 2016−2022 (project no. 2014-2020.4.01.16.0032). the Foundation for Polish Science TEAM NET POIR.04.04.00-00-16ED/18-00
dc.identifier.citationAnal. Chem. 2023, 95, 2, 1574–1581 Publication Date:January 4, 2023 https://doi.org/10.1021/acs.analchem.2c04644en
dc.identifier.doi10.1021/acs.analchem.2c04644
dc.identifier.issn1520-6882
dc.identifier.urihttps://depot.ceon.pl/handle/123456789/22221
dc.language.isoen
dc.publisherAmerican Chemical Societyen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectmicrofluidicsen
dc.subjectdropletsen
dc.subjectdroplet microfluidicsen
dc.subjectantibioticsen
dc.subjectmolecular transporten
dc.titlePhysicochemical Properties Predict Retention of Antibiotics in Water-in-Oil Dropletsen
dc.typearticleen
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