Ammonium nanochelators in conjunction with arginine‑specific enzymes in amperometric biosensors for arginine assay
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58_2024.Ammonium nanochelators in conjunction with arginine-specific enzymes in amperometric biosensors for arginine assay_OA.pdf (2.42 MB)
0000-0002-6135-3294 Date
2023-12-22
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Springer Nature
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Abstract
Amino acid L-arginine (Arg), usually presented in food products and biological liquids, can serve both as a useful indicator of food quality and an important biomarker in medicine. The biosensors based on Arg-selective enzymes are the most promising devices for Arg assay. In this research, three types of amperometric biosensors have been fabricated. They exploit arginine oxidase (ArgO), recombinant arginase I (ARG)/urease, and arginine deiminase (ADI) coupled with the ammonium-chelating redox-active nanoparticles. Cadmium-copper nanoparticles (nCdCu) as the most effective nanochelators were used for the development of ammonium chemosensors and enzyme-coupled Arg biosensors. The fabricated enzyme/nCdCu-containing bioelectrodes show wide linear ranges (up to 200 µM), satisfactory storage stabilities (14 days), and high sensitivities (A⋅M−1⋅m−2) to Arg: 1650, 1700, and 4500 for ADI-, ArgO- and ARG/urease-based sensors, respectively. All biosensors have been exploited to estimate Arg content in commercial juices. The obtained data correlate well with the values obtained by the reference method. A hypothetic scheme for mechanism of action of ammonium nanochelators in electron transfer reaction on the arginine-sensing electrodes has been proposed.
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Stasyuk, N., Gayda, G., Nogala, W. et al. Ammonium nanochelators in conjunction with arginine-specific enzymes in amperometric biosensors for arginine assay. Microchim Acta 191, 47 (2024). https://doi.org/10.1007/s00604-023-06114-1