In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis

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dc.contributor.authorChornobrov, Oksana
dc.contributor.authorBilous, Svitlana
dc.contributor.organizationNational University of Life and Environmental Sciences of Ukraine, Boyarka Forestry Research Station, Plant Biotechnology Research Laboratory, Boyarka, Ukraine
dc.contributor.organizationNational University of Life and Environmental Sciences of Ukraine, Botany, Dendrology and Forest Tree Breeding Department, Kyiv, Ukraine
dc.contributor.organizationInstitute for Evolutionary Ecology NAS Ukraine, Department of Dendrology and Park Learning, Kyiv, Ukraine
dc.date.accessioned2024-08-23T08:53:05Z
dc.date.available2024-08-23T08:53:05Z
dc.date.issued2021-03-15
dc.description.abstractPlants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).en
dc.identifier.citationChornobrov, Oksana and Bilous, Svitlana. "In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis" Folia Forestalia Polonica, vol.63, no.1, 2021, pp.68-73. https://doi.org/10.2478/ffp-2021-0007
dc.identifier.doi10.2478/ffp-2021-0007
dc.identifier.issn2199-5907
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/24732
dc.language.isoen
dc.publisherCommittee on Forestry Sciences and Wood Technology of the Polish Academy of Sciences and the Forest Research Institute in Sękocin Stary
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Międzynarodoween
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceFolia Forestalia Polonica, Series A – Forestry
dc.subjectChristmas cactusen
dc.subjectnutrient mediumen
dc.subjectexplanten
dc.subjectcallusen
dc.subjectshoot proliferationen
dc.subjectmicroclonal propagationen
dc.titleIn vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesisen
dc.typearticle
dc.type.versionpublishedVersion
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