Artificial nitrate reductase-based electrodes: A dual-function approach for self-powered explosive-selective sensors and biofuel cells
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71_2025.Artificial nitrate reductase-based electrodes. A dual-function approach for self-powered explosive-selective sensors and biofuel cells.pdf (7.58 MB)
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0000-0001-6550-8145 Date
2025-09-16
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Elsevier
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Miniaturized devices powered by fuel cells for the detection of explosives would play an important role in enhancing safety and security in military operations, improving public safety measures, facilitating environmental cleanup in contaminated areas, and supporting humanitarian efforts in post-conflict regions. Amperometric sensors based on artificial enzymes are efficient and cost-effective tools for a variety of analytical applications. In the current study, bio(chemo)sensors based on highly selective artificial nitrate reductases or natural nitrate reductase for detecting 2,4,6-trinitrotoluene (TNT) and 1,3,5-trinitro-1,3,5-triazinane (hexogen, RDX) in post-explosive soil samples, as well as related biofuel cells (BFCs), have been developed. The fabricated amperometric bio(chemo)sensors based on cadmium‑cobalt nanocomposites and copper nanostructures possess high sensitivity (6330 A⋅M− 1⋅m− 2 and 6070 A⋅M− 1⋅m− 2), low limit of detection and good selectivity towards the target analytes, TNT and RDX. The developed best BFC generates an open circuit potential of 560 mV with a maximum power density of 0.850 μW⋅cm− 2 at an optimum of 0.1 mM RDX. The constructed biofuel cells were tested for model bioremediation on the real samples of the post-explosive soils.
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Chemical Engineering Journal 523 (2025) 168560