Point-of-care testing for C-reactive protein in a sequential microfluidic device

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dc.contributor.authorBoonkaew, Suchanat
dc.contributor.authorSzot-Karpińska, Katarzyna
dc.contributor.authorNiedziółka-Jönsson, Joanna
dc.contributor.authorPałys, Barbara
dc.contributor.authorJönsson-Niedziółka, Martin
dc.contributor.organizationInstitute of Physical Chemistry, Polish Academy of Sciencesen
dc.contributor.organizationFaculty of Chemistry, University of Warsawen
dc.date.accessioned2023-10-23T12:41:40Z
dc.date.available2023-10-23T12:41:40Z
dc.date.issued2023
dc.description.abstractPoint-of-care testing (POCT) devices are currently available for the detection of various important diseases owing to their easy-to-use, portable, and user-friendly nature. However, the multistep regent manipulation still impedes the performance of the device for end-users. To address this issue, we present a novel sequential flow-through microfluidic device that incorporates dual flow behaviors (fast-flow/delayed) into a single device for assessing the risk of cardiovascular disease (CVDs) using C-reactive protein (CRP) as the model analyte. The device features a fast-flow channel for automated washing of unbound CRP antigens and a delayed channel for flow of a redox reagent for electrochemical analysis. It requires only a single buffer loading, resulting in a total analysis time of 15 min. To overcome challenges related to storage stability and the affinity of antibodies in the manufacturing process, we integrated the P3-CRP peptide as a new bioreceptor for CRP detection. The performance of both the P3-CRP peptide and antibodies was assessed and examined using chronocoulometry. The proposed device exhibited a limit of detection at 47 pg mL−1 with a wide linear range (5 orders of magnitude). To validate its applicability, the device was tested with serum, plasma, and whole blood samples, yielding satisfactory results.en
dc.description.sponsorshipThis research was supported by the National Centre for Research and Development (NCBR) through the EEA and Norway Grants (Project number: NOR/POLNOR/UPTURN/0060/2019) and the Polish National Science Centre via a SONATA 13 grant UMO-2017/26/D/ST5/00980en
dc.identifier.citationSensors Actuat B: Chemical 397, 134659. https://doi.org/10.1016/j.snb.2023.134659en
dc.identifier.doi10.1016/j.snb.2023.134659
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/22975
dc.language.isoen
dc.publisherElsevieren
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectpoint-of-care devicesen
dc.subjectpeptidesen
dc.subjectmicrofluidicsen
dc.subjectphage displayen
dc.subjectC-reactive proteinen
dc.subjectlabel-free immunoassayen
dc.subjectcapillary-driven microfluidic deviceen
dc.subjectelectrochemical detectionen
dc.titlePoint-of-care testing for C-reactive protein in a sequential microfluidic deviceen
dc.typearticleen
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