Modified glucose as a sensor to track the metabolism of individual living endothelial cells - Observation of the 1602 cm-1 band called “Raman spectroscopic signature of life”

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dc.contributor.authorPieczara, Anna
dc.contributor.authorBorek-Dorosz, Aleksandra
dc.contributor.authorBuda, Szymon
dc.contributor.authorTipping, William
dc.contributor.authorGraham, Duncan
dc.contributor.authorPawlowski, Robert
dc.contributor.authorMlynarski, Jacek
dc.contributor.authorBaranska, Malgorzata
dc.contributor.organizationJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian Universityen
dc.contributor.organizationJagiellonian University in Kraków, Doctoral School of Exact and Natural Sciences, Polanden
dc.contributor.organizationFaculty of Chemistry, Jagiellonian Universityen
dc.contributor.organizationCentre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, United Kingdomen
dc.contributor.organizationInstitute of Organic Chemistry, Polish Academy of Sciencesen
dc.date.accessioned2023-08-01T13:06:29Z
dc.date.available2023-08-01T13:06:29Z
dc.date.issued2023
dc.description.abstractA relatively new approach to subcellular research is Raman microscopy with the application of sensors called Raman probes. This paper describes the use of the sensitive and specific Raman probe, 3-O-propargyl-D-glucose (3-OPG), to track metabolic changes in endothelial cells (ECs). ECs play a significant role in a healthy and dysfunctional state, the latter is correlated with a range of lifestyle diseases, particularly with cardiovascular disorders. The metabolism and glucose uptake may reflect the physiopathological conditions and cell activity correlated with energy utilization. To study metabolic changes at the subcellular level the glucose analogue, 3-OPG was used, which shows a characteristic and intense Raman band at 2124 cm-1. 3-OPG was applied as a sensor to track both, its accumulation in live and fixed ECs and then metabolism in normal and inflamed ECs, by employing two spectroscopic techniques, i.e. spontaneous and stimulated Raman scattering microscopies. The results indicate that 3-OPG is a sensitive sensor to follow glucose metabolism, manifested by the Raman band of 1602 cm-1. The 1602 cm-1 band has been called the “Raman spectroscopic signature of life” in the cell literature, and here we demonstrate that it is attributed to glucose metabolites. Additionally, we have shown that glucose metabolism and its uptake are slowed down in the cellular inflammation. We showed that Raman spectroscopy can be classified as metabolomics, and its uniqueness lies in the fact that it allows the analysis of the processes of a single living cell. Gaining further knowledge on metabolic changes in the endothelium, especially in pathological conditions, may help in identifying markers of cellular dysfunction, and more broadly in cell phenotyping, better understanding of the mechanism of disease development and searching for new treatments.en
dc.description.sponsorshipNational Science Center, Poland (NCN) (OPUS15 no. UMO-2018/29/B/ST4/00335); Visibility&Mobility module under the program “Excellence Initiative – Research University” at the Jagiellonian University in Krakow; grant from the Faculty of Chemistry under the Strategic Programme Excellence Initiative at Jagiellonian University
dc.identifier.citationBiosensors and Bioelectronics 230 (2023) 115234 ; https://doi.org/10.1016/j.bios.2023.115234en
dc.identifier.doi10.1016/j.bios.2023.115234
dc.identifier.issn0956-5663
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/22786
dc.language.isoen
dc.publisherElsevieren
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject3-OPG glucoseen
dc.subjectendotheliumen
dc.subjectspontaneous Raman microscopyen
dc.subjectstimulated Raman scatteringen
dc.titleModified glucose as a sensor to track the metabolism of individual living endothelial cells - Observation of the 1602 cm-1 band called “Raman spectroscopic signature of life”en
dc.typearticleen
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