The effect of coconut extract on callus growth and ultrasound waves on production of betulin and betulinic acid in in-vitro culture conditions of Betula pendula Roth species

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dc.contributor.authorPayamnoor, Vahide
dc.contributor.authorHajati, Razieh Jafari
dc.contributor.authorKhodadai, Negar
dc.contributor.organizationShahed University, Traditional Clinical Trial Research Center, Tehran, Iranen
dc.contributor.organizationGorgan University of Agricultural Sciences and Natural Resources, Faculty of Forest Sciences, Gorgan, Iranen
dc.date.accessioned2019-01-04T13:00:05Z
dc.date.available2019-01-04T13:00:05Z
dc.date.issued2018-12-15
dc.description.abstractTo determine the effect of coconut extract on callogenesis of Betula pendula, Roth stem barks were cultured in NT (Nagata and Takebe) basic culture media in two individual experiments: i) cultivation explant in different treatments of coconut extracts combined with 1 mg l-1 2, 4-D (2, 4-Dichlorophenoxyacetic acid) and ii) callogenesis in NT media containing 1.5 mg l-1 2,4-D and 0.5 mg l-1 BAP (6-Benzylaminopurine) and then cultivation under the first experiment treatments. The first experiment demonstrated that not all concentrations of coconut extracts lead to callus induction individually, but callus induction increased 84% in a culture containing 5% coconut extract plus 1 mg l-1 2, 4-D. Based on the results of the second experiment, this treatment also significantly increased the wet and dry weights of the produced calluses. The possibility of increasing the betulinic acid and betulin by ultrasound was also studied. Samples cultivated in the selected culture medium were exposed to ultrasound waves in two forms of 1) one exposure and 2) twice exposure (repetition with 24 hr interval) in steps of 20, 60, 100, and 160 sec, and one treatment as the control. Betulin and the betulinic acid amount were determined using HPLC (High-Performance Liquid Chromatography). The maximum betulinic acid and betulin were obtained in 100 sec in once exposure (2.3 mg g-1 DW) and 160 sec in the twice exposure (0.75 mg g-1 DW) to ultrasound. The results showed that betulinic acid content was more than betulin.en
dc.identifier.citationPayamnoor, V. Hajati, R.J. & Khodadai, N. (2018). The effect of coconut extract on callus growth and ultrasound waves on production of betulin and betulinic acid in in-vitro culture conditions of Betula pendula Roth species. Folia Forestalia Polonica, Series A – Forestry, 60 (4), 261–268en
dc.identifier.doi10.2478/ffp-2018-0027
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/16291
dc.language.isoen
dc.publisherThe Committee on Forestry Sciences and Wood Technology of the Polish Academy of Sciences and the Forest Research Institute in Sekocin Staryen
dc.rightsUznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/*
dc.subjectbetulinen
dc.subjectbetulinic aciden
dc.subjectBetula pendula Rothen
dc.subjectcallus inductionen
dc.titleThe effect of coconut extract on callus growth and ultrasound waves on production of betulin and betulinic acid in in-vitro culture conditions of Betula pendula Roth speciesen
dc.typearticleen
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