Wheat germ lipase: isolation, purification and applications

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Authors
Kublicki, Marcin
Koszelewski, Dominik
Brodzka, Anna
Ostaszewski, Ryszard
Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
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Abstract
In recent years, wheat germ lipase (WGL) is attracting considerable interest. To date, several WGL applications have already been described: (i) fats and oils modification; (ii) esterification reactions in organic media, accepting a wide range of acids and alcohols as substrates; (iii) the asymmetric resolution of various chiral racemic intermediates; (iv) more recently, the promiscuous activity of WGL has been shown in carbon-carbon bond formation. To date, no crystallographic structure of this enzyme has been published, which means its activity, catalytic potential and substrate scope is being assessed empirically. Therefore, new catalytic activities of this enzyme are constantly being discovered. Taking into account the emergency and the current interest in environmentally sustainable processes, this review aims to highlight the origin, isolation, stabilization by immobilization and applications of the wheat germ lipase.
Description
Keywords
wheat germ lipase  immobilization  esterification  hydrolysis  enzymatic resolution  enzyme promiscuity
Citation
Marcin Kublicki, Dominik Koszelewski, Anna Brodzka & Ryszard Ostaszewski (2022) Wheat germ lipase: isolation, purification and applications, Critical Reviews in Biotechnology, 42:2, 184-200, DOI: 10.1080/07388551.2021.1939259
URI
https://open.icm.edu.pl/handle/123456789/21832
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