In vitro effects of polychlorinated biphenyls and their hydroxylated metabolites on the synthesis and metabolism of iodothyronines in the chicken (Gallus domesticus) thyroid gland

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dc.contributor.authorKowalik, Kinga
dc.contributor.authorSechman, Andrzej
dc.contributor.organizationUniversity of Agriculture in Krakow, Department of Animal Physiology and Endocrinology
dc.date.accessioned2024-04-29T12:25:50Z
dc.date.available2024-04-29T12:25:50Z
dc.date.issued2022-02-10
dc.description.abstractTo assess the effect of polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) on thyroid hormone [TH: thyroxine (T4) and triiodothyronine (T3)] secretion, the concentrations of iodothyronine deiodinases (DIO1, DIO2, DIO3), and mRNA expression of genes involved in TH synthesis (TSHR, NIS, TPO, TG), metabolism (DIO1, DIO2, DIO3), and transport (OATP1C1, MCT8, MCT10, LAT1), chicken thyroid explants were incubated in medium supplemented with TSH (250 mU/ml), PCB118, PCB153, 4-OH-PCB107, and 3-OH-PCB153 (0.5 × 10-8 M), and TSH together with each PCB and OH-PCB. The results of the in vitro experiment revealed that, except for 4-OH-PCB107, all applied PCBs and OH-PCBs inhibited basal and TSH-stimulated T4 secretion. Moreover, they increased basal and reduced TSH-stimulated T3 secretion. PCBs and OH-PCBs decreased the TSH-stimulated TSHR expression. Following PCB and OH-PCB exposure, significant changes in mRNA expression of NIS, TPO, and TG were observed. PCBs and OH-PCBs affected DIO1 and DIO3 transcript levels and protein abundances of each DIO. Furthermore, PCB-dependent effects on OATP1C1, MCT8, and MCT10 mRNA expression were found. In conclusion, both PCB118 and PCB153 and their OH-PCBs affect TH synthesis and deiodination processes in the chicken thyroid gland and influence TH transport across the thyrocyte membrane. In addition, the effects of PCBs and OH-PCBs depended mainly on the type of PCB congener and the exposure time. These results indicate that not only parental PCBs but also OH-PCBs are hazardous for the thyroid gland and may disrupt its endocrine function. Further studies are necessary to explain a mechanism of PCB and OH-PCB action in the avian thyroid gland.
dc.description.sponsorshipThis work was supported by the National Science Centre, Poland, under Grant [number UMO-2017/27/N/NZ9/01733].
dc.identifier.citationGeneral and Comparative Endocrinology 318 (2022) 113989. https://doi.org/10.1016/j.ygcen.2022.113989.
dc.identifier.doi10.1016/j.ygcen.2022.113989
dc.identifier.issn0016-6480
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/24224
dc.language.isoen
dc.publisherELSEVIER
dc.relation.ispartofseries318; 113989
dc.rightsDozwolony użytek
dc.sourceGeneral and Comparative Endocrinology
dc.subjectpolychlorinated biphenyls
dc.subjectPCB hydroxylated metabolites
dc.subjectthyroid hormone
dc.subjectthyroid gland
dc.subjectchicken
dc.titleIn vitro effects of polychlorinated biphenyls and their hydroxylated metabolites on the synthesis and metabolism of iodothyronines in the chicken (Gallus domesticus) thyroid glanden
dc.title.alternativeEfekty in vitro polichlorowanych bifenyli i ich hydroksylowanych metabolitów na syntezę i metabolizm jodotyronin w tarczycy kury (Gallus domesticus)pl
dc.typearticle
dc.type.versionpublishedVersion
person.identifier.orcidKowalik, Kinga [0000-0001-6921-5569]
person.identifier.orcidSechman, Andrzej [0000-0002-8377-7100]
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