Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis

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dc.contributor.authorLipko, Agata
dc.contributor.authorPączkowski, Cezary
dc.contributor.authorPerez-Fons, Laura
dc.contributor.authorFraser, Paul D.
dc.contributor.authorKania, Magdalena
dc.contributor.authorHoffman-Sommer, Marta
dc.contributor.authorDanikiewicz, Witold
dc.contributor.authorRohmer, Michel
dc.contributor.authorPoznanski, Jaroslaw
dc.contributor.authorSwiezewska, Ewa
dc.contributor.organizationInstitute of Biochemistry and Biophysics, Polish Academy of Sciencesen
dc.contributor.organizationDepartment of Plant Biochemistry, Faculty of Biology, University of Warsawen
dc.contributor.organizationSchool of Biological Sciences, Royal Holloway, University of Londonen
dc.contributor.organizationInstitute of Organic Chemistry, Polish Academy of Sciencesen
dc.contributor.organizationUniversité de Strasbourg/CNRS, Institut Le Bel, Strasbourg, Franceen
dc.date.accessioned2024-01-08T14:05:10Z
dc.date.available2024-01-08T14:05:10Z
dc.date.issued2023
dc.description.abstractIsoprenoids, including dolichols (Dols) and polyprenols (Prens), are ubiquitous components of eukaryotic cells. In plant cells, there are two pathways that produce precursors utilized for isoprenoid biosynthesis: the mevalonate (MVA) pathway and the methylerythritol phosphate (MEP) pathway. In this work, the contribution of these two pathways to the biosynthesis of Prens and Dols was addressed using an in planta experimental model. Treatment of plants with pathway-specific inhibitors and analysis of the effects of various light conditions indicated distinct biosynthetic origin of Prens and Dols. Feeding with deuteriated, pathway-specific precursors revealed that Dols, present in leaves and roots, were derived from both MEP and MVA pathways and their relative contributions were modulated in response to precursor availability. In contrast, Prens, present in leaves, were almost exclusively synthesized via the MEP pathway. Furthermore, results obtained using a newly introduced here ‘competitive’ labeling method, designed so as to neutralize the imbalance of metabolic flow resulting from feeding with a single pathway-specific precursor, suggest that under these experimental conditions one fraction of Prens and Dols is synthesized solely from endogenous precursors (deoxyxylulose or mevalonate), while the other fraction is synthesized concomitantly from endogenous and exogenous precursors. Additionally, this report describes a novel methodology for quantitative separation of 2H and 13C distributions observed for isotopologues of metabolically labeled isoprenoids. Collectively, these in planta results show that Dol biosynthesis, which uses both pathways, is significantly modulated depending on pathway productivity, while Prens are consistently derived from the MEP pathway.en
dc.description.sponsorshipNational Science Center of Poland [UMO-2018/29/B/NZ3/01033] ; International PhD Projects Program of the Foundation for Polish Science (grant MPD/2009-3/2) ; University of London, COST Action FA1006 — Plant Metabolic Engineering for High Value Products
dc.identifier.citationBiochemical Journal (2023) 480 495–520 ; https://doi.org/10.1042/BCJ20220578en
dc.identifier.doi10.1042/BCJ20220578
dc.identifier.issn0006-2960
dc.identifier.issn1520-4995
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/23521
dc.language.isoen
dc.publisherPortland Pressen
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDivergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsispl
dc.typearticleen
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