Combining rotary wet-spinning biofabrication and electro-mechanical stimulation for the in vitro production of functional myo-substitutes

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dc.contributor.authorCelikkin, Nehar
dc.contributor.authorPresutti, Dario
dc.contributor.authorMaiullari, Fabio
dc.contributor.authorVolpi, Marina
dc.contributor.authorPromovych, Yurii
dc.contributor.authorGizynski, Konrad
dc.contributor.authorDolinska, Joanna
dc.contributor.authorWiśniewska, Agnieszka
dc.contributor.authorOpałło, Marcin
dc.contributor.authorParadiso, Alessia
dc.contributor.authorRinoldi, Chiara
dc.contributor.authorFuoco, Claudia
dc.contributor.authorSwieszkowski, Wojciech
dc.contributor.authorBearzi, Claudia
dc.contributor.authorRizzi, Roberto
dc.contributor.authorGargioli, Cesare
dc.contributor.authorCostantini, Marco
dc.contributor.organizationInstitute of Physical Chemistry, Polish Academy of Sciencesen
dc.contributor.organizationIstituto Nazionale Genetica Molecolare INGM 'Romeo ed Enrica Invernizzi', Milan, Italyen
dc.contributor.organizationPhD Program in Cellular and Molecular Biology, Department of Biology, University of Rome Tor Vergata, Rome, Italyen
dc.contributor.organizationFaculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Polanden
dc.contributor.organizationDepartment of Biology, University of Rome, Tor Vergata, Rome, Italyen
dc.contributor.organizationDepartment of Medical Surgical Sciences and Biotechnologies, Sapienza University of Rome, Rome, Italyen
dc.contributor.organizationInstitute of Biomedical Technologies, National Research Council of Italy (ITB-CNR), Milan, Italyen
dc.date.accessioned2023-12-14T14:45:04Z
dc.date.available2023-12-14T14:45:04Z
dc.date.issued2023-08-09
dc.description.abstractIn this work, we present an innovative, high-throughput rotary wet-spinning biofabrication method for manufacturing cellularized constructs composed of highly-aligned hydrogel fibers. The platform is supported by an innovative microfluidic printing head (MPH) bearing a crosslinking bath microtank with a co-axial nozzle placed at the bottom of it for the immediate gelation of extruded core/shell fibers. After a thorough characterization and optimization of the new MPH and the fiber deposition parameters, we demonstrate the suitability of the proposed system for the in vitro engineering of functional myo-substitutes. The samples produced through the described approach were first characterized in vitro and then used as a substrate to ascertain the effects of electro-mechanical stimulation on myogenic maturation. Of note, we found a characteristic gene expression modulation of fast (MyH1), intermediate (MyH2), and slow (MyH7) twitching myosin heavy chain isoforms, depending on the applied stimulation protocol. This feature should be further investigated in the future to biofabricate engineered myo-substitutes with specific functionalities.en
dc.description.sponsorshipThis study was supported by the National Science Centre Poland (NCN) within the SONATA 14 2018/31/D/ST8/03647 and PRELUDIUM 19 Project No. 2020/37/N/ST5/03272. This work was also supported by Research Projects CNR Progetti at CNR under the Grant No.: CUP: B93C20046330005. We would like to acknowledge Prof Fabio Domenici at Department of Chemical Science and Technologies, University of Rome "Tor Vergata", Rome, Italy for their support in confocal image acquisition.en
dc.identifier.citationCelikkin, Nehar, et al. "Combining rotary wet-spinning biofabrication and electro-mechanical stimulation for the in vitro production of functional myo-substitutes." Biofabrication 15.4 (2023): 045012.en
dc.identifier.doi10.1088/1758-5090/ace934
dc.identifier.issn1758-5090
dc.identifier.urihttps://open.icm.edu.pl/handle/123456789/23389
dc.language.isoen
dc.publisherIOPen
dc.rightsUznanie autorstwa 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject3D bioprintingen
dc.subjectskeletal muscle tissue engineeringen
dc.subjectmechanical stimulationen
dc.subjectelectrical stimulationen
dc.subjectbioreactoren
dc.titleCombining rotary wet-spinning biofabrication and electro-mechanical stimulation for the in vitro production of functional myo-substitutesen
dc.typearticleen
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